https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Paradigm shift in frog sperm cryopreservation: reduced role for non-penetrating cryoprotectants https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53343 50% for each). Theoretically, it has been suggested that increased NPCP concentration should improve cell survival by increasing the rate and extent of cell dehydration. We suggest, however, that the elevated osmolality in the unfrozen water fraction when 10% sucrose is used may be causing damage to cells via excessive cell shrinkage and solute effects as proposed in the two-factor hypothesis of cryoinjury. We showed this response in sperm across a range of frog species, providing compelling evidence for this hypothesis. We suggest protocol development using the PCP/NPCP ratios demonstrated in our study will be broadly applicable to many amphibian species.]]> Wed 22 Nov 2023 10:19:22 AEDT ]]> Deconstructing compassionate conservation https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35503 Wed 19 Aug 2020 11:21:51 AEST ]]> Adhesive defence mucus secretions in the red triangle slug (Triboniophorus graeffei) can incapacitate adult frogs https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36887 Wed 15 Jul 2020 10:53:42 AEST ]]> The Increasing Role of Short-Term Sperm Storage and Cryopreservation in Conserving Threatened Amphibian Species https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51284 Wed 06 Mar 2024 15:39:43 AEDT ]]> Generation of reproductively mature offspring from the endangered green and golden bell frog Litoria aurea using cryopreserved spermatozoa https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49076 Wed 03 May 2023 16:15:16 AEST ]]> Sperm collection and storage for the sustainable management of amphibian biodiversity https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37426 Tue 17 Nov 2020 10:22:21 AEDT ]]> Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34867 Tue 03 Sep 2019 18:01:56 AEST ]]> Development of sperm cryopreservation and assisted reproductive technologies for the conservation of threatened Australian tree frogs https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53557 Thu 14 Mar 2024 08:46:39 AEDT ]]> Differential success in obtaining gametes between male and female Australian temperate frogs by hormonal induction: a review https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42613 Mon 22 May 2023 10:52:38 AEST ]]> Refrigerated storage and cryopreservation of hormonally induced sperm in the threatened frog, Litoria aurea https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54771 50%) when preserved with 15% v/v DMSO and 1% w/v sucrose; and (3) spermic urine stored at 5 ◦C retained motility for up to 14 days. Our findings demonstrate that the protocols developed in this study allowed for successful induction and recovery of high-quality spermatozoa from a threatened Australian anuran, L. aurea, providing a prime example of how ARTs can contribute to the conservation of rare and threatened species.]]> Mon 11 Mar 2024 15:07:50 AEDT ]]> Tadpole Fingerprinting: Using Tail Venation Patterns to Photo-Identify Tadpole Individuals of a Threatened Frog https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50519 Mon 07 Aug 2023 13:26:18 AEST ]]> Cryopreservation Cooling Rate Impacts Post-Thaw Sperm Motility and Survival in Litoria booroolongensis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54088 Mon 05 Feb 2024 09:21:29 AEDT ]]> Efficacy of short-term cold storage prior to cryopreservation of spermatozoa in a threatened lizard https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49400 Fri 12 May 2023 14:41:06 AEST ]]> A model protocol for the cryopreservation and recovery of motile lizard sperm using the phosphodiesterase inhibitor caffeine https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38175 Fri 06 Aug 2021 14:14:50 AEST ]]> Sperm cryopreservation in an Australian skink (Eulamprus quoyii) https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49168 Fri 05 May 2023 15:37:01 AEST ]]>